Category: Jobs

PhD: Developing novel biosensors for monitoring antibody production in CHO cells

The biopharmaceutical market is valued at over 100 billion dollars per year with the majority of therapeutic antibodies being manufactured in Chinese hamster ovary (CHO) cells. However, the process of generating monoclonal antibody (mAb) producing cell lines suitable for commercial manufacture remains challenging, time consuming and costly.

The cellular pathways underpinning antibody production in CHO cells have been extensively studied over the past 10 years; however, it is still unclear which pathways are most important for this process (protein folding, protein transport and cell stress). The aim of this PhD is to facilitate a deeper understanding of CHO mAb production cell lines by investigating the intracellular phenotypes of CHO cell lines which are producing and secreting monoclonal antibodies. Using the knowledge gained we aim to define a phenotypic fingerprint which correlates with favourable manufacturing and product quality characteristics. Once these fingerprints have been defined, a series of novel fluorescent tools for monitoring them will be developed and applied to our cell line development platform to allow screening for optimal mAb producing cell lines in the earliest stages of cell line development.

This project will provide training in advanced mammalian cell culture, fluorescence microscopy, flow cytometry and access to state of the art, high-throughput, automated systems for cell culture (Berkeley Lights Beacon) and phenotypic analysis (Intellicyt iQue)   in place at the GSK laboratories.

The studentship is available starting in October 2017. This project is collaboration between Dr Andrew Peden at the University of Sheffield and Dr Robyn Emmins, GSK Biopharmaceutical Process Research, Stevenage. Candidates should have a strong academic degree in the biological sciences. The successful candidate will be highly motivated, work effectively in a team setting and be interested in cell biology, protein trafficking and technology development.

For an informal discussion regarding the post, please contact:
Dr Andrew Peden
Dr Robyn Emmins

This is a BBSRC-funded Industrial CASE PhD studentship and is full time for four years. The stipend will be £17,553.00 per annum. Applicant eligibility criteria for the studentship can be found at www.bbsrc.ac.uk/documents/studentship-eligibility-pdf/ and you must identify that you fit these criteria prior to application.

Business Development Manager – Suprex

Suprex Ltd (www.suprex.uk), which specialises in the development of processes using supercritical carbon dioxide, is seeking a Business Development Manager to build new relationships primarily in the natural products sector and to manage the Company’s grant applications. We are looking for a commercially focused scientist with a Masters or PhD in Chemistry or related subject with experience of business development in a science based field. Knowledge of supercritical carbon dioxide theory and uses would be advantageous but can be provided.
For further details contact Andy Beggin andy@suprex.uk.

Relevant Skills
·  A commercially focused scientist with a Masters or PhD in chemistry or related subject
·  Experience of business development preferably in a science based field
·  Experience of writing grant applications (e.g. Innovate UK, Horizon 2020) would be highly advantageous
·  Ability to communicate, written and verbal, with both researchers and commercial contacts will be critical
·  Must be able to work under own initiative and to demanding deadlines
·  Knowledge of supercritical carbon dioxide theory and uses would be advantageous but can be provided

Principal Scientist – Mammalian Cell Culture; Fujifilm Diosynth Biotechnologies

Fujifilm Diosynth Biotechnologies is a world leading cGMP contract development and manufacturing organisation. We currently have over 530 employees at our Billingham site and are looking to expand within R&D.  We have an exciting opportunity for a highly motivated scientist to join our R&D Mammalian Cell Culture team working to develop recombinant cell lines suitable for the manufacture of biotherapeutics.

The successful candidate will be laboratory-based and, working as part of a team, will be involved in cell line development (CLD; from transfection, cloning and selection of cell lines to evaluation of clonal cell lines in scale-down mimics of the production process). They will also have a role to play in contributing to the implementation of new and innovative ways of working.

Technical Skills
In depth Knowledge of CLD for mammalian cell lines (ideally CHO)
Good aseptic technique and knowledge of general cell culture methods
Experience of transfection and cloning techniques
Experience with high throughput cell line screening technologies, such as the ClonePix and ambr systems.

Education and experience
A life science degree (Ideally a MSc or PhD)
Relevant biotech/pharmaceutical industry experience within a mammalian cell culture role
Experience in developing stable cell lines for the production of therapeutic proteins using CHO cells

For further information please contact Fay Saunders
Closing date: 23rd June 2017