Category: Jobs

D.Phil position at Oxford / Pirbright / Horizon Discovery

An exciting D.Phil position is available at the Pirbright Institute in Guildford, with a placement at Horizon Discovery and support from the Oxford University Doctoral Training Partnership.

Transcriptional Analysis of chIFITM knockout technology for increased vaccine yields
http://www.pirbright.ac.uk/postgraduate-studentship-opportunities/studentships-2017

Start date: October 2017; Remuneration/ pay rate: £17,003 studentship stipend
Supervisors:
Dr Mark Fife, Dr Angela Steyn, The Pirbright Institute
Prof. Adrian Smith, Department of Zoology, University of Oxford
Dr Jon Moore, Horizon Discovery

Contact details:
mark.fife@pirbright.ac.uk Tel: +44 (0)1483 232441

Avian viruses create major challenges to poultry health through loss of productivity and mortality & have concomitant effects on the global poultry industry through a reduction in the output of poultry meat & eggs. Developing effective and affordable vaccines against these viral diseases will help to increase food security worldwide & alleviate poverty in developing countries. This studentship will focus on the transcriptional analysis of CRISPR/Cas9 chIFITM gene knock-outs in commercially relevant and lab-adapted avian cell lines, and will aim to prove the hypothesis that reduced/ablation of chIFITM gene expression will result in a significant increase in viral replication and augment vaccine viral titre.

The position is open to biological science graduates with at least a 2.1 or equivalent, or a Masters degree (subject to university regulations). Other first degrees, e.g. veterinary science, will be considered. You should be looking for a challenging, interdisciplinary research training environment and have an active interest in the control of infectious diseases.

Several vacancies at F-star

F-star develops therapeutic bi-specific antibodies using its Modular Antibody Technology, directed at improving the treatment of serious diseases, with a focus on immune-oncology. We are entering an exciting period of the Company’s development and have several vacancies at the Babraham Research Campus in Cambridge, UK.

Research associate – protein sciences
You will be responsible for protein purification and subsequent routine characterisation, filtrations and sterility testing of antigens, antibody fragments and bi-specific mAb2 molecules.
• A relevant degree and/or a combination of education and experience
• Good track record of relevant hands-on, lab-based experience
• Protein purification using AKTA system (antibody purification, ion exchange, affinity chromatography; eg IMAC)
• Protein characterisation (HPLC, SDS-PAGE, endotoxin level testing etc.)
• Relevant industry experience

Research associate – molecular biology
You will be responsible for the design and execution of expression vector cloning to express antibodies and fusion proteins in mammalian systems
• Relevant experience ideally gained in an industrial environment
• Hands-on experience in molecular biology techniques and vector design for expression of antibodies in mammalian systems (DNA manipulation, cloning, sequence alignment and analysis)
• Knowledge in antibody engineering (eg isotype/subtype reformatting)

Research associate – mammalian cell culture
You will be responsible for transient and stable expression of recombinant proteins in mammalian cell lines at several lab scales.
• Relevant industry or academic experience in mammalian cell culture
• Hands-on experience in transient expression of recombinant proteins using mammalian cell systems (HEK293 and CHO)
• Knowledge in CHO cell line development including transfection, marker selection and generation of stable pools will be an advantage

Scientist – protein characterisation
you will proactively contribute to the development and delivery of analytical capabilities to support our technology platform and mAb2 bispecific pipeline.  Analytical capabilities are required to deliver protein engineering, research grade production and process development activities with a key focus on protein characterisation, developability assessments and formulation
– PhD or equivalent relevant experience, ideally gained in an industrial environment although consideration will be given to industrially relevant academic experience
– Experience and breadth of knowledge in analytical and biophysical characterisation of recombinant biotherapeutics for CMC development. (e.g. formulation, protein aggregation, protein stability and post-translational modifications including glycosylation)
– Experience in early stage developability assessment of therapeutic proteins and development lifecycle for recombinant protein therapeutics
– Demonstrated experience in establishing and applying the following techniques: dynamic and static light scattering, capillary electrophoresis, SVP, UHPLC (SEC, RP, IEX), mass spectrometry

Further details of these and other exciting roles along with instructions how to apply can be found at http://www.f-star.com/careers

PhD: Developing novel biosensors for monitoring antibody production in CHO cells

The biopharmaceutical market is valued at over 100 billion dollars per year with the majority of therapeutic antibodies being manufactured in Chinese hamster ovary (CHO) cells. However, the process of generating monoclonal antibody (mAb) producing cell lines suitable for commercial manufacture remains challenging, time consuming and costly.

The cellular pathways underpinning antibody production in CHO cells have been extensively studied over the past 10 years; however, it is still unclear which pathways are most important for this process (protein folding, protein transport and cell stress). The aim of this PhD is to facilitate a deeper understanding of CHO mAb production cell lines by investigating the intracellular phenotypes of CHO cell lines which are producing and secreting monoclonal antibodies. Using the knowledge gained we aim to define a phenotypic fingerprint which correlates with favourable manufacturing and product quality characteristics. Once these fingerprints have been defined, a series of novel fluorescent tools for monitoring them will be developed and applied to our cell line development platform to allow screening for optimal mAb producing cell lines in the earliest stages of cell line development.

This project will provide training in advanced mammalian cell culture, fluorescence microscopy, flow cytometry and access to state of the art, high-throughput, automated systems for cell culture (Berkeley Lights Beacon) and phenotypic analysis (Intellicyt iQue)   in place at the GSK laboratories.

The studentship is available starting in October 2017. This project is collaboration between Dr Andrew Peden at the University of Sheffield and Dr Robyn Emmins, GSK Biopharmaceutical Process Research, Stevenage. Candidates should have a strong academic degree in the biological sciences. The successful candidate will be highly motivated, work effectively in a team setting and be interested in cell biology, protein trafficking and technology development.

For an informal discussion regarding the post, please contact:
Dr Andrew Peden
Dr Robyn Emmins

This is a BBSRC-funded Industrial CASE PhD studentship and is full time for four years. The stipend will be £17,553.00 per annum. Applicant eligibility criteria for the studentship can be found at www.bbsrc.ac.uk/documents/studentship-eligibility-pdf/ and you must identify that you fit these criteria prior to application.

Business Development Manager – Suprex

Suprex Ltd (www.suprex.uk), which specialises in the development of processes using supercritical carbon dioxide, is seeking a Business Development Manager to build new relationships primarily in the natural products sector and to manage the Company’s grant applications. We are looking for a commercially focused scientist with a Masters or PhD in Chemistry or related subject with experience of business development in a science based field. Knowledge of supercritical carbon dioxide theory and uses would be advantageous but can be provided.
For further details contact Andy Beggin andy@suprex.uk.

Relevant Skills
·  A commercially focused scientist with a Masters or PhD in chemistry or related subject
·  Experience of business development preferably in a science based field
·  Experience of writing grant applications (e.g. Innovate UK, Horizon 2020) would be highly advantageous
·  Ability to communicate, written and verbal, with both researchers and commercial contacts will be critical
·  Must be able to work under own initiative and to demanding deadlines
·  Knowledge of supercritical carbon dioxide theory and uses would be advantageous but can be provided