Dr Jody Mason’s lab at the University of Bath is looking for a bright and enthusiastic individual who is interested in using a new and cutting edge peptide library screening approach to derive functional peptide-based antagonists of alpha-Synuclein (aS) associated toxicity. The project is wide-ranging and will involve extensive molecular biology, peptide synthesis, and protein biochemistry to derive functional antagonists and to characterise their mechanism of action (see Cheruvara et al., J Biol Chem 2015). The project is highly collaborative and will be undertaken in collaboration with partners at Bath and the University of Queensland to provide a detailed picture of how antagonists function, including their ability to block aS associated toxicity both in vitro and within live neuronal cells, The project will require travel in the UK and possibly beyond.
Our amyloid-antagonist selection assay is fully developed and this interdisciplinary project now seeks to build and screen libraries and to understand how our functional antagonists of work and to generate second generation inhibitors, and multi-mode inhibitors and mimetics. In parallel, our collaborators in the Williams group at Bath will use extensive cell-biology using primary neuronal cell lines to demonstrate that molecules are functional, non-toxic, cell-penetrant, and to establish the precise molecular mechanism of action inside disease-related cells. This component of the project will be supported by a dedicated technician. Using this information we will work together to generate better inhibitors. This is an exciting and hugely valuable project, for which training and support will be given throughout, including regular meetings between team members and the possibility to travel and gain new skills.
The Mason lab is very well-equipped with all necessary equipment dedicated to the project including assay development, automated peptide synthesis, protein purification, biophysical analysis, and cell-biology. In addition, the University houses numerous shared facilities from NMR and Mass Spectrometry to cell biology and super-resolution imaging.
This 3-yr Alzheimer’s Research UK funded Postdoctoral position is available from 1st September and applicants must be highly motivated and hold a PhD in a relevant science subject. Candidates should also have experience in molecular cloning and protein biochemistry/biophysics. Knowledge of peptide library screening would be advantageous. For more information or informal enquiries please contact Dr. Jody Mason: +44 1225 386867. https://researchportal.bath.ac.uk/en/persons/jody-mason