Investigating the cutaneous lipidome to understand how skin responds to sunlight exposure
We want to explore the UVR-induced changes in skin lipids as means of understanding the normal physiology of human skin when exposed to sunlight, and appreciate the role of lipids in maintaining skin health. We will then use this information to understand how human skin reacts with xenobiotics that interact with sunlight and, this way, support the design of studies for the development of pharmaceuticals. Overall, the project will allow detailed study and understanding of the skin lipidome and perturbations induced by sunlight and xenobiotics, permit mapping of lipid networks contributing to inflammatory responses and inform research into human health.
We will use 3D human skin models, primary skin cells in culture, and human skin in organ culture. We will then employ mass spectrometry-based assays and lipid imaging to assess changes in the various types of skin lipids and their accumulation in cell membranes.
The project will provide training in multiple disciplines (biochemistry, analytics, pharmaceutics, biology) including the handling of large data sets generated by lipidomics. While at UoM, the student will train in cell and organ culture, lipid extractions and analyses by mass spectrometry (triple quadrupole and time-of-flight coupled to liquid and supercritical fluid chromatography), and data processing. During the placement periods, the student will learn the requirements of drug development and how skin physiology and photo reactivity can inform drug discovery. The student will also have access to AstraZeneca’s research into microphysiological systems and 3D cell models that will be used to investigate aspects of normal skin functioning, and will work with AstraZeneca’s world leading mass spectrometry imaging group to investigate novel ways to detect changes in the lipidome.
This is a CASE studentship is to be funded under the BBSRC Doctoral Training Programme. If you are interested in this project, please make direct contact with the Principal Supervisor to arrange to discuss the project further as soon as possible. You MUST also submit an online application form – full details on how to apply can be found on the BBSRC DTP website.