PDRA position: functionally active peptide inhibitors of transcription factor activity

Dr Jody Mason’s lab at the University of Bath is looking for a bright and enthusiastic individual who is interested in using a new and cutting edge peptide library screening approach to derive functional peptide-based antagonists of transcription factor activity. The project is wide-ranging and will involve extensive molecular biology, peptide synthesis, and protein biochemistry to derive functional antagonists and to characterise their mechanism of action (see Baxter et al. ACS Chem Biol, 2017). The project is highly collaborative and will be undertaken in collaboration with partners in the UK and the University of Queensland to provide a detailed picture of how antagonists function, including their ability to block transcription of target genes, The project will require travel in the UK and possibly beyond.

Our antagonist selection assay is fully developed and this interdisciplinary project now seeks to build and screen libraries and to understand how identified functional antagonists of transcription factor proto-oncogenes work. In parallel, our collaborators in the Kad group at the University of Kent will use single molecule imaging to directly visualise RNA polymerase moving along DNA and learn how inhibitors exert their action. Using this information we will work together to generate better inhibitors. This is an exciting and hugely valuable project, for which training and support will be given throughout, including regular meetings between team members and a 6-week exchange programme with Kent to gain new skills and enhance progress.

The Mason lab is very well-equipped with all necessary equipment dedicated to the project including automated peptide synthesis, protein purification, biophysical analysis, and cell-biology. In addition, the University houses numerous shared facilities from NMR and mass spectrometry to cell biology and super-resolution imaging.

This 3-yr BBSRC funded postdoctoral position is available from 1st August and applicants must be highly motivated and hold a PhD in a relevant science subject. Candidates should also have experience in molecular cloning and protein biochemistry/biophysics. Knowledge of peptide library screening would be advantageous. For more information or informal enquiries please contact Dr. Jody Mason: j.mason@bath.ac.uk +44 1225 386867. https://researchportal.bath.ac.uk/en/persons/jody-mason

Application deadline is 15th July 2018. Apply now: https://tinyurl.com/MasonLab-BBSRC-PDRA